The dots are placed based on the day of illness (x-axis) and something called cycle threshold, which is a measure of the number of cycles in PCR that it took to detect viral RNA (y-axis). The misconception related to this paper was that people thought it proved that ADE could occur after receipt of the COVID-19 vaccine. Whether our results are true for other cell types in humans, or if they are specific to mRNA vaccines, are among many questions for further research. The persistence of the spike protein in the body raises the prospect of sustained inflammation within and damage to organs which express the spike protein, according to experts atDoctors for COVID Ethics,an organization consisting of physicians and scientists seeking to uphold medical ethics, patient safety, and human rights in response to COVID-19.. Childrens Hospital of Philadelphia is a charitable 501(c)(3) nonprofit organization. (AP Photo/Jae C. Hong, File), Connect with the definitive source for global and local news, A clip being shared online in recent days shows three doctors, who have spread misinformation about the vaccines in the past, discussing the Swedish study and falsely claiming it demonstrates that the Pfizer vaccine reverse transcribes and installs DNA into the human genome, leading to harmful effects. WebImmunogenicity after SARS-CoV-2 vaccination is known to be impaired in liver transplant (LT) recipients, but the results after the application of a third dose show significant improvement in seroconversion rates. Organisation number: 202100-3211, Webmail Finally, as the authors pointed out, expression of LINE-1 has been shown to increase during viral infections, including with SARS-CoV-2 virus. -. The browser you are using is not supported by this website. Importantly, LINE-1 has been associated with various disease-related conditions, including cancer. The data represent RNA levels not viral particles, so while the patient samples required equal cycles of PCR to detect RNA, we cant speak to a persons contagiousness because this study does not tell us how much whole virus was present in their nasal passages. They have two things in common. Andy B. on LinkedIn: Pfizer Vaccine Indeed, about 8 percent of human DNA comes from viruses inserted into our genomes during evolution. 5 Notably, mutations in the BRCA tumor-suppressor genes can be inherited. Both boys were pronounced dead at home. YDM: About 18 percent of the vaccine accumulates in the liver just 30 minutes after the vaccine is injected in mice as reported by Pfizer in EMA assessment report, and therefore we chose to study liver cells. The pharmaceutical industry is dangerous to health. Cells that received no vaccine at all were used for control purposes. Clipboard, Search History, and several other advanced features are temporarily unavailable. In the general population, the antibody response wanes over the course of time after two doses of the vaccination, but seems to be more Serves: 4 Prep. In the case of Covid shots, the vaccines from Moderna and Pfizer ( PFE )/BioNTech introduce a single spike protein. WebIf proto-oncogenes or tumor suppressor genes mutate, cells can become cancerous. The result might be surprising, but it is also a bit surprising that such studies do not seem to have been carried out before. Below Australian experts address this concern Expert Comments: Professor Nigel McMillan Program Director of Infectious Diseases and Immunology at the Menzies Health Institute Queensland , Griffith 2021 Dec 22;9(3):e0116221. At this stage, we do not know if DNA reverse transcribed from BNT162b2 is integrated into the cell genome. 2020;383:26032615. Your email address is used only to let the recipient know who sent the email. and transmitted securely. A new Swedish study published in MDPI found that the Pfizer vaccine goes into liver cells and converts to DNA, challenging claims so far that the mRNA COVID-19 vaccines do not change or interact Safety and Immunogenicity of Two RNA-Based COVID-19 Vaccine Candidates. The Study Has Received A Lot of Media Attention, What Are Your Thoughts on That? And even if there was, what are you going to do with 147 kids' data?7 Based on this study, is there any reason to not get vaccinated? 2022 Apr;28(4):612.e1-612.e7. (In-vitro Swedish Study) https://youtube.com/live/MjxlvduyJyc?feature=share 28 Feb 2023 02:39:58 followers 129 videos. It has also been shown to affect immune responses. Surg Neurol Int. We show that the vaccine enters liver cells as That is why it is impossible to alter your DNA with mRNA vaccines. A new study from Lund University in Sweden on how the Pfizer-BioNTech COVID-19 vaccine affects human liver cells under experimental conditions, has been viewed more than 800,000 times in just over a week. Further, the newest vaccines are personalized, meaning they can target a patients doi: 10.1056/NEJMoa2034577. The whole process of Pfizers mRNA transfecting liver cells occurred within just six hours, per the study. Together, theyre working to enrol as many as 10,000 patients in a trial of new mRNA cancer vaccines, starting this year. No added sugar, full of protein and chocolate! 5. Finally, protein shapes change in different environments, so while it may be interesting to compare in which parts of the body these proteins with similar stretches are located, we cant really know whether cross-reactivity would be an issue simply by comparing the gene sequences or even by working with the proteins on the lab bench. 2022 Nov;28(11):2273-2287. doi: 10.1038/s41591-022-02061-1. The result might be surprising, but it is also a bit surprising that such studies do not seem to have been carried out before. J. Med. Accessibility It is known from animal studies that it is also expressed early in embryonic development", explains Yang de Marinis. The experiment was conducted in a petri dish and aimed to answer the question of whether the mRNA in the vaccine could be converted to DNA. Some of the vaccine dose is going to make it into the bloodstream, of course. The authors of a Swedish study said their study could not be used to draw conclusions with human DNA as they used cell lines in a laboratory petri dish for their work. Two people in their 60s who received the shot were diagnosed with Guillain-Barre syndrome, out of about 20,000 vaccine recipients who participated in Pfizers Phase 3 Bethesda (MD): National Institute of Child Health and Human Development; 2006. (In-vitro Swedish Study) https://youtube.com/live/MjxlvduyJyc?feature=share 28 Feb 2023 02:39:58 They obtained more than 100 vials of the vaccine and tested them They found that 99% was Graphene Oxide (GO). Two different antibody-dependent enhancement (ADE) risks for SARS-CoV-2 antibodies. If you dont have time (or interest) in reviewing the primary source of the information, it is often best to let the information die in your feed or your inbox. Pfizer Vaccine Becomes DNA in Liver Cells. This site uses cookies to assist with navigation, analyse your use of our services, collect data for ads personalisation and provide content from third parties. We show that the vaccine enters liver cells as early as 6 hours after the vaccine has been administered. Pfizer did not comment on the studys findings, the Epoch Times reported, stating only that its mRNA vaccine does not alter the human genome. We also show that BNT162b2 mRNA is reverse transcribed intracellularly into DNA in as fast as 6 h upon BNT162b2 exposure. We detected high levels of BNT162b2 in Huh7 cells and changes in gene expression of long interspersed nuclear element-1 (LINE-1), which is an endogenous reverse transcriptase. Well do this by highlighting a few papers that caused fear, generated confusion, or spread misinformation by looking at the interpretation, what it was based on, and why it was not correct. Since Hypothesis and Theory articles in this journal do not require methods or results sections, this paper met the criteria for publication. Preclinical studies of COVID-19 mRNA vaccine BNT162b2, developed by Pfizer and BioNTech, showed reversible hepatic effects in animals that received the The PubMed wordmark and PubMed logo are registered trademarks of the U.S. Department of Health and Human Services (HHS). 8600 Rockville Pike 2022 Oct 21;13:475. doi: 10.25259/SNI_377_2022. In the August 2022 James Gill paper titled Autopsy Histopathologic Cardiac Findings in 2 adolescents following the second COVID-19 vaccine dose, two teenage boys died in their sleep within the first week after receiving the 2nd Pfizer COVID-19 mRNA vaccine dose. This document is subject to copyright. Click here:\rhttps://www.drbeen.com/yt-special-p/\r\rWant to support this work? eCollection 2022 Dec. Mavridis C, Aidonidis G, Evangelou M, Kalogeridis A. Hist Philos Life Sci. "LINE-1 can convert RNA to DNA and has been shown to be found in tissues, including stem cells, in the human body. APS ASSESSMENT: False. While that point alone is enough to confirm that this paper does not offer evidence that COVID-19 vaccines cause ADE, it is also worth noting that the Methods section of the paper contains only five sentences, and the author describes looking at online protein banks and comparing the SARS-CoV-2 spike protein sequence to sequences of other proteins. Scientists estimate that the spike protein, like other proteins our bodies create, may stay in the body up to a few weeks., Further, the CDC says on a web page titled Myths and Facts about COVID-19 Vaccines that the genetic material delivered by mRNA vaccines never enters the nucleus of your cells.. Our bodies also have tens, if not hundreds, of thousands of different proteins. In a study published in Cell Reports, researchers from Lund, Patients are often asked to rate their feelings using a rating scale, when talking to psychologists or doctors about their mental health. Indeed, if you look at the article in the journal in which it was published. This also explains the choice of vaccine concentrations in our study, something we specifically address in the paper, which are 0.5-2% of the injection site concentration. time: 15 Cooks in: 1:40 Ready in: 1:55 Ingredients 1 kg beef flatiron 4 tbsp extra-virgin olive oil 1 garlic clove, finely chopped 1 tbsp tomato paste cup []. The mean time from COVID-19 diagnosis to cholangiopathy was 118 days. Webhttps://lnkd.in/dSz2cbQJ This was what everyone told me would not happen- that the RNA vaccines would not go into the DNA! And, again, while this journal allows for presenting a theory, the authors choice to use sections suggestive of a research study (methods, results, discussion) is somewhat misleading. Spike proteins may circulate in the body after an infection or injection with a COVID-19 vaccine. The study has received a lot of media attention, what are your thoughts on that? Two of the authors, Associate Professor Yang de Marinis (YDM) and Professor Magnus Rasmussen (MR), share their views. According to Swedish researchers at Lund University, the messenger mRNA from Pfizers Covid vaccine can enter human liver cells and is converted into DNA. The research was conducted at Lund University. Reverse transcription is when DNA is made from RNA, whereas the normal transcription process involves a portion of the DNA serving as a template to make an mRNA molecule inside the nucleus. Invasion of Ukraine: Russia and Ukraine ready for second round of talks tonight. Huh7 cells were exposed to BNT162b2, and quantitative PCR was performed on RNA extracted from the cells. But keep in mind, when the mRNA or adenovirus particles do hit cells outside of the liver or the site of injection, they're still causing them to express Spike protein anchored on their surfaces, not dumping it into the circulation. Some viruses, like HIV, are known to be able to convert RNA to DNA and then incorporate that DNA into host cells genome, which is one thing that makes the illness difficult to treat. The views expressed in this article are those of the author and do not reflect the official position of yourNEWS. Each dot on the table represents a study subject. Evaluating information: What you should know, Dissecting social media: What you should know. Keywords: MR: There is no reason for anyone to change their decision to take the vaccine based on this study. In interpreting this data, some people chose to focus only on the similar values in the first few days of the curve and use that to suggest that vaccinated people were equally likely to spread the virus compared with unvaccinated people. Nature. Rasmussen also said, cell lines differ from cells in living organisms, and therefore it is important that similar investigations are also studied in humans.. 2021;385:759760. PMC About BVHS; Mayo Clinic Care Network; News & Media; Contact Us The author described two potential mechanisms by which this could occur. Daily science news on research developments and the latest scientific innovations, The latest engineering, electronics and technology advances, The most comprehensive sci-tech news coverage on the web. Immunohistochemistry using antibody binding to LINE-1 open reading frame-1 RNA-binding protein (ORFp1) on Huh7 cells treated with BNT162b2 indicated increased nucleus distribution of LINE-1. Salesforce has already slashed 8,000 workers from its ranks this year. BNT162b2 mRNA is reverse transcribed intracellularly into DNA as fast as 6 [hours] after BNT162b2 exposure.. Moore also cautioned that the cells used in the study were quite different than primary cells in the body. The cell line came from liver cancer cells, which means their replication mechanisms were more active, and amplified the process in a way that would not have happened in healthier human cells, Moore said. Click here to sign in with This is the first time that researchers have shown in vitro or inside a petri dish how an mRNA vaccine is converted into DNA on a human liver cell line, and is what health experts and fact-checkers said for over a year couldnt occur. First, cancerous cell lines replicate, whereas our liver cells typically are not replicating. The .gov means its official. If you take just one thing away from this series, we hope you will have come to realize that science does not easily fit into a sound bite and, most often, it is not an either-or proposition, so if something sounds too simple, you probably need to ask more questions. To understand why this is unlikely, one needs to know a bit about protein chemistry: An analysis like the one in this paper may allow one to create an interesting hypothesis, but it does not provide evidence of a concern. This article was produced by the Reuters Fact Check team. Would you like email updates of new search results? The most delicious sticker pack has been created for theolivemagazine.grcommunity on Rakuten Viber, Man dressed as giant penis arrested in Rio de Janeiro carnival, Three Greek destinations in the top 20 for holidays in Europe in 2023, Elon Musk Recommends Investing In Physical Things Here Are 3 Physical Assets That Perform Well During High Inflation, Zlatans first Padel club to open in Greece, NASA confirms half-ton meteor crashed in South Texas (video), The State of Democracy Where is Greece (infographic), The Worlds Oldest Populations Where is Greece? A cancer vaccine seemed a far-fetched idea just a few years ago, but now the shots exist and are gaining steam in the biotech community. Furthermore, a recent study showed that SARS-CoV-2 RNA can be reverse-transcribed and integrated into the genome of human cells. 2021 Jun 7;22(5):172. doi: 10.1208/s12249-021-02058-y. You should not consider the information in this site to be specific, professional medical advice for your personal health or for your family's personal health. The https:// ensures that you are connecting to the In the study, the authors infected a human liver cell line with the Pfizer version of the COVID-19 vaccine. Once the string of amino acids is created in the cell, chemical and physical forces, such as the charges and shapes of the amino acids in the sequence as well as other factors in the cellular environment, cause the newly formed protein to fold into a 3D shape. 2022 Apr 11;44(4):1661-1663. doi: 10.3390/cimb44040113. Bad Bunny. The. Cells that received no vaccine at all were used for control purposes.The researchers then investigated how different gene expressions in the cells changed over time. And, while this may be the case early during infection, there are a few additional considerations when thinking about these data: Hopefully these examples demonstrated some of the ways that a paper should be examined. Another aspect of protein chemistry is also important. J. Med. Bethesda, MD 20894, Web Policies Disclaimer. A segment of the protein called the open reading frame-1, or ORF-1, then goes back into the nucleus, where it attaches to the vaccines mRNA and reverse transcribes into spike DNA. YDM: This study does not investigate whether the Pfizer vaccine alters our genome. sharing sensitive information, make sure youre on a federal For these reasons, while the changes related to LINE-1 are interesting, we cant be sure the effects would be the same in a non-cancerous cell line. Missing context. Likewise, even if these data were found to be representative of ones ability to spread the virus, it is not appropriate to dismiss the rest of the curve, which shows differences between vaccinated and unvaccinated people after day four or five. PCR on genomic DNA of Huh7 cells exposed to BNT162b2 amplified the DNA sequence unique to BNT162b2. The content provided in this video is for informational and educational purposes only.\rPlease consult with a physician or healthcare professional regarding any medical or mental health related diagnosis or treatment. Khehra N, Padda I, Jaferi U, Atwal H, Narain S, Parmar MS. AAPS PharmSciTech. Pfizers BNT162b2 mRNA Covid-19 vaccine was the first vaccine against the novel coronavirus (COVID-19) approved for distribution in the United States. The vaccines mRNA converting into DNA and being found inside the cells nucleus is something that the Centers for Disease Control and Prevention (CDC) said would not happen. CMAJ. Virological and serological kinetics of SARS-CoV-2 Delta variant vaccine breakthrough infections: a multicentre cohort study. Press Office However, this is not an appropriate conclusion from this paper for several reasons: Logical fallacy called hasty generalization (It is important to note in this case, that the misconception was not because of the quality of the science or the messaging of the authors, but rather because others took the findings out of context.). N. Engl. The two that have made the news recently are the Pfizer vaccine and the Moderna vaccine. Lund Universitymagnus [dot] rasmussen [at] med [dot] lu [dot] se, Yang de Marinis WebPfizer Vaccine Becomes DNA in Liver Cells. These cells have had the normal protections of the immune system removed.. PCR primer set used to detect mRNA level and reverse-transcription of BNT162b2. Original video "Pfizer Vaccine Becomes DNA in Liver Cells. 2023 Jan 4;45(1):400-433. doi: 10.3390/cimb45010028. The study tested whether the vaccines Huh7, MeSH in petri dishes, the researchers added Pfizer BioNTech's vaccine to a cell line that originally came from a human liver tumour. This condition is not common, but as the author indicates, concerns about ADE have arisen for other coronaviruses. The clip comes from a longer roundtable discussion that was posted March 8 by Christian broadcasting network Daystar, during which faith leaders discussed COVID-19 vaccines with the doctors. Huh7 cells were treated, Immunohistochemistry of Huh7 cells treated, Immunohistochemistry of Huh7 cells treated with BNT162b2 on LINE-1 protein distribution. Reuters has previously fact checked the claim that mRNA vaccines alter human DNA (here), (here), (here) and (here). 2020;383:24392450. The whole process of Pfizers mRNA transfecting liver cells occurred within just six hours, per the study. In this case, the author compared gene sequences to identify sections of human proteins that have portions similar to SARS-CoV-2 proteins and made a prediction that the similarities would cause immune responses against oneself. This is important for two reasons; both of which were acknowledged by the authors. A clip being shared online in recent days shows three doctors, who have spread misinformation about the vaccines in the past, discussing the Swedish study and falsely claiming it demonstrates that the Pfizer vaccine reverse transcribes and installs DNA into the human genome, leading to harmful effects. The study authors clarified their research in a Q&A in March after it received significant attention, stating this study does not investigate whether the Pfizer vaccine alters our genome, and that there is no reason for anyone to change their decision to take the vaccine based on this study.. But studies are showing that is not the case. The mRNA then leaves the nucleus and enters the cells cytoplasm, where it translates into LINE-1 protein. The messenger RNA (mRNA) from Pfizer s COVID-19 5 Notably, mutations in the BRCA tumor-suppressor genes can be inherited. official website and that any information you provide is encrypted "LINE-1 can convert RNA to DNA and has been shown to be found in tissues, including stem cells, in the human body. A Clinical Update on SARS-CoV-2: Pathology and Development of Potential Inhibitors. Genomics, diabetes and endocrinologyyang [dot] de_marinis [at] med [dot] lu [dot] se, Box 117, 221 00 Lund, Sweden 19.9M. So, one may quickly realize that with 20 building blocks and tens or hundreds of thousands of proteins, small (sometimes even large) stretches of overlap are likely to occur. YDM: More research is needed. https://www.microsoft.com/en-us/microsoft-365/windows/end-of-ie-support). 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Meaning they can target a patients doi: 10.3390/cimb45010028 and serological kinetics of SARS-CoV-2 Delta pfizer vaccine becomes dna in liver vaccine infections! Mrna then leaves the nucleus and enters the cells U, Atwal h, Narain,. Theyre working to enrol as many as 10,000 patients in a trial of new Search results at the article the. 2022 Oct 21 ; 13:475. doi: 10.1056/NEJMoa2034577 paper met the criteria for publication not require or. N, Padda I, Jaferi U, Atwal h, Narain S, Parmar MS. PharmSciTech! Target a patients doi: 10.3390/cimb45010028 13:475. doi: 10.25259/SNI_377_2022 trial of new mRNA cancer vaccines, this... Original video `` Pfizer vaccine Indeed, about 8 percent of human cells proteins may in!
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